Documentation



Singleton et al.

LIBSHUFF Home

LIBSHUFF Theory

SIMO Project

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webLIBSHUFF version 0.96

This webpage will allow you to compare two libraries of 16S rRNA gene sequences and determine if they are significantly different. This service is provided by the Sapelo Island Microbial Observatory project, funded by the National Science Foundation.

This script will run PRELIBSHUFF and LIBSHUFF version 1.22 on a PHYLIP-format distance matrix file and email the results to you. Please see the "Documentation" link to the left for instructions, and the LIBSHUFF homepage for more information on the original program.

webLIBSHUFF is written by James R. Henriksen (contact for technical problems), LIBSHUFF by Dr. Dave Singleton, and PRELIBSHUFF by Rima Upchurch.

The path to the distance matrix file:
This file should be an unmodified DNADIST output file.

How many sequences are in the first library (X)?
What is the name of the first library (X)?

How many sequences are in the second library (Y)?
What is the name of the second library (Y)?

What is the email that you would like the results emailed to?

Please double check the information above, particularly the email address. A webLIBSHUFF run with one hundred sequences in each library will take approximately two minutes, while a webLIBSHUFF run with four hundred sequences in each library will take approximately two hours. Libraries over 2000 sequences are not allowed at this time.

The Sapelo Island Microbial Observatory is funded by the National Science Foundation

NSF

This material is based upon work supported by the National Science Foundation under Cooperative Agreement #MCB-0084164. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

THIS SOFTWARE IS PROVIDED BY THE AUTHORS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE AUTHORS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.